Terminator

Part:BBa_K3789006:Design

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-26)


ADH1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Differently from bacterial terminators, S. cerevisiae terminators contain a hexamer sequence, an efficiency element, that strongly resembles the eukaryotic TATA box i.e. the promoter sequence recognized and bound by RNA polymerase II. ADH1 terminator is used as an expression-enhancing terminator to increase mRNA half-life and improve gene expression control for metabolic engineering applications.



Source

The sequence we used comes from Saccharomyces cerevisiae S288c.

References